• University Theses (Thèses d'agrégation de l'enseignement supérieur)
  Holders have performed at least 4-10 years of post-doctoral work and have published serveral original and review papers of excellent quality on their research topic. They are considered as experts in their field in a broad sense. Awarding of the title is made by the Rector of University upon recommendation of an ad-hoc committee and after (i) the formal submission of a wtitten Thesis, supported by review papers; ii) the formal defense of the Thesis, together with 3 subsidiary theses (Thèses annexes); (iii) the formal presentation of a Public Lecture on a topic decided on by the Jury.
  
  
• PhD Theses (Thèses de Doctorat)
  Holders have presented an original experimental work supported by the publication of at least two original papers in Scientifc Journals of an impact factor of at least 2. The presentation is made accordign a two steps procedure (i) a private defense for the Jury only; (ii) a public defense and the formal publication of the Thesis.
  
  
• DEA (Diplôme d'Etudes Approfondies)
  Holders are graduated students (i.e. holders of a MSc. degree or equivalent) who have (i) performed a one-year research activity of a defined topic, (ii) have submitted a formal wtitten document (Mémoire), and (iii) have presented their data in front of a Jury. Some of these students may have published original papers in Scientifc Journals
  
  
• Master Degree Dissertations (Mémoires de fins d'études)
  Holders are young graduating students who (i) have performed a one-year research activities as part of their university studies; (ii) have presented their data in front of a Jury. Master Dissertation is compulsory for students registered (i) with the Faculty of Science (all orientations); (ii) in the Faculty of Medicine (orientation: Biomedical Sciences). It may also be presented by students in Pharmacy as part of their activities as "research students"

• Thesis
  • Full text (15 MB)
  • Slides (Public Defense and Public Lesson)
    • Main Thesis: WEB - PDF
      
    • Subsidiary theses (thèses annexes)
      • Inhibiting efflux pumps to restore antibiotic activity against Pseudomonas aeruginosa: WEB - PDF
      • Daptomycin: a new-old antibiotic or how did pharmacodynamics bring back to life a disappointing drug ?: WEB - PDF
      • Cholesterol transporters as new therapeutic targets: WEB - PDF
    • Public Lesson (in French): L'importance de la vie intracellulaire dans les infections bactériennes (PDF - 32 MB)
      
      
• Publications (reviews)
• Glycopeptide antibiotics: from conventional molecules to new derivatives (UCL only; copyright retained by the Publisher)
  Drugs (2004) 64:913-936
  
• Antibiotic efflux pumps in prokaryotic cells : occurence, impact on resistance and strategies for the future of antimicrobial therapy (UCL only; copyright retained by the Publisher)
  Journal of Antimicrobial Chemotherapy (2003) 51:1055-1065
  
• Antibiotic efflux pumps in eukaryotic cells: occurence and impact on antibiotic cellular pharmacokinetics, pharmacodynamics and toxicodynamics (UCL only; copyright retained by the Publisher)
  Journal of Antimicrobial Chemotherapy (2003) 51:1067-1077

Marie-Paule Mingeot-Leclercq (2000)

Biochemical and biophysical studies of the interactions between aminoglycoside antibiotics and lipid layers: a molecular approach to the understanding of the cellular toxicity induced by aminoglycoside antibiotics

• Slides (Public defense)
  
  
• Publications (reviews)
  • Aminoglycosides: activity and resistance (UCL only; copyright retained by the Publisher)
    Antimicrob Agents Chemother (1999) 43:727-737.
  • Aminoglycosides: nephrotoxicity (UCL only; copyright retained by the Publisher)
    Antimicrobial Agents and Chemotherapy (1999) 43:1003-1012.
  • Molecular parameters involved in aminoglycosides nephrotoxicity.
    Journal of Toxicology and Environmental Health (1995) 44:263-300.
  • Molecular description of the interactions of aminoglycoside antibiotics with negatively-charged phospholipids. Theoretical molecular modelling and experimental results.
    Life Science/Advances in Pharmacology (1995) 14:71-98.

• Publications (review):
  • Aminoglycoside-induced phospholipidosis and nephrotoxicity.
    Biochemical Pharmacology (1990) 40:2383-2392.
  • Kidney tissue repair after nephrotoxic injury : biochemical and morphological characterization.
    CRC Critical Review of Toxicolology (1988) 19:147-183.
  • Aminoglycoside nephrotoxicity: cellular and molecular aspects.
    In: ISI Atlas of Science: Pharmacology, Waverly Press, Baltimore, 40-44 (1987)
    

Perrin Ngougni Pokem (2022)

Clinical pharmacokinetcis of temocillin: A way to optimize personalized dosing

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  
  
• Publications
  • Binding of temocillin to plasma proteins in vitro and in vivo: the importance of plasma protein levels in different populations and of co-medications
    J Antimicrob Chemother 2022; 77(10):2742-2753
  • Population Pharmacokinetics of Temocillin Administered by Continuous Infusion in Patients with Septic Shock Associated with Intra-Abdominal Infection and Ascitic Fluid Effusion
    Antibiotics 2022 5;11(7):898

Gang Wang (2022)

Reviving disused antibiotics against Gram-negative ESKAPE pathogens: combination with the polyaminoisoprenyl potentiator NV716

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  
  
• Publications
  • The membrane-active polyaminoisoprenyl compound NV716 re-sensitizes Pseudomonas aeruginosa to antibiotics and reduces bacterial virulence
    Communications Biology 2022; 5(1):871.
  • The polyamino-isoprenyl potentiator NV716 revives disused antibiotics against Gram-negative bacteria in broth, infected monocytes, or biofilms, by disturbing the barrier effect of their outer membrane
    European Journal of Medicinal Chemistry 2022; 238:114496.
  • The polyaminoisoprenyl potentiator NV716 revives old disused antibiotics against intracellular forms of infection by Pseudomonas aeruginosa
    Antimicrobial Agents and Chemotherapy 2021; 65(3):e02028-20.

Albert Ruiz Sorribas (2021)

Targeting inter-kingdom biofilms with antimicrobials and hydrolytic enzymes

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  
  
• Publications
  • Hydrolytic enzymes as potentiators of antimicrobials against an inter-kingdom biofilm model
    Microbiology Spectrum - 2022; 10(1):e0258921
  • Pharmacodynamics of moxifloxacin, meropenem, caspofungin and their combinations against in vitro polymicrobial inter-kingdom biofilms
    Antimicrobial Agents and Chemothery 2022; 6(2):e02149-21.
  • In vitro polymicrobial inter-kingdom three-species biofilm model: influence of hyphae on biofilm formation and bacterial physiology.
    Biofouling 2021; 37(5):481-493

Hervé Poilvache (2021)

:In-vitro and in-vivo study of biofilm disruption strategies for the treatment of Prosthetic Joint Infections.

PhD in Medical Sciences (Promotor: O. Cornu, IREC; Co-promotor, F. Van Bambeke)

• Thesis
  • Full text
  
  
• Publications
  • In-vitro study of the synergistic effect of an enzyme cocktail and antibiotics against biofilms in a prosthetic joint infection model
    Antimicrobial Agents and Chemotherapy 2021; 65(4):e01699-20
  • Synergistic effects of pulsed lavage and antimicrobial therapy against Staphylococcus aureus biofilms in an in-vitro model
    Frontiers in Medicine (2020) 7:527
  • Development of an innovative in vivo model of PJI treated with DAIR.
    Frontiers in Medicine (2022) 9:984814.

Frédéric Peyrusson (2021)

Intracellular persistence of Staphylococcus aureus: from observations to mechanisms

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  
  
• Publications
  • In Vitro Models for the Study of the Intracellular Activity of Antibiotics
    In Bacterial Persistence: Methods and Protocols (Second edition)
  • Host cell oxidative stress induces dormant Staphylococcus aureus persisters
    Microbiology Spectrum 10(1):e0231321
    
  • Intracellular Staphylococcus aureus persisters upon antibiotic exposure.
    Nature Communications (2020) 11:2200 (14 printed pages)
  • Cellular pharmacokinetics and intracellular activity of the bacterial fatty acid synthesis inhibitor afabicin desphosphono against different resistance phenotypes of Staphylococcus aureus in models of cultured phagocytic cells
    International Journal of Antimicrobial Agents (2020) 55:105848 (9 printed pages)
  • Cellular pharmacokinetics and intracellular activity of gepotidacin against Staphylococcus aureus with different resistance phenotypes in models of cultured phagocytic cells
    Antimicrobial Agents and Chemotherapy (2018) 27;62:e02245-17
  • Cellular pharmacokinetics and intracellular activity of the novel peptide deformylase inhibitor GSK1322322 against Staphylococcus aureus laboratory and clinical strains with various resistance phenotypes. Studies with human THP-1 monocytes and J774 murine macrophages
    Antimicrobial Agents and Chemotherapy (2015) 59:5747-5760

Yvan Diaz Iglesias (2020)

Activity of antibiotics against in vitro mono-species biofilms of P. aeruginosa and S. aureus in the context of cystic fibrosis: The influence of the culture medium

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)

   

• Publications
  • Activity of antibiotics against Staphylococcus aureus in an in vitro model of biofilms in the context of cystic fibrosis: influence of the culture medium
    Antimicrobial Agents and Chemotherapy (2019) 63:e00602-19 (14 printed pages)
  • Activity of antibiotics against Pseudomonas aeruginosa in an in vitro model of biofilms in the context of cystic fibrosis: influence of the culture medium
    Antimicrobial Agents and Chemotherapy (2020) 64:e02204-19 (14 printed pages)

Tiep Khac Nguyen (2020)

Antibiotic resistance in Vietnam in relation with persistent forms of infection

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Public Defense (slides)

   

• Publications
  • Antibiotic resistance, biofilm formation, and intracellular survival as possible determinants of persistent or recurrent infections by Staphylococcus aureus in a Vietnamese tertiary hospital. Focus on bacterial response to moxifloxacin.
    Microbial Drug Resistance (2020) 26(6):537-544
  • The persister character of clinical isolates of Staphylococcus aureus contributes to faster evolution to resistance and higher survival in THP-1 monocytes: a study with moxifloxacin.
    Frontiers in Microbiology (2020) 11:587364 (12 printed pages)

Jules César Bayiha (2020)

Characterization of the effects of the budesonide-hydroxypropyl-ß-cyclodextrin complex on lipid membranes and the cellular response to hydrogen peroxide and lipopolysaccharide

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)

   

• Publications
  • The budesonide-hydroxypropyl-ß-cyclodextrin complex attenuates ROS generation, IL-8 release and cell death induced by oxidant and inflammatory stress. Study on A549 and A-THP-1 cells.
    Molecules. (2020) 25:4882 (21 printed pages)
  • Changes in membrane biophysical properties induced by the Budesonide/Hydroxypropyl-ß-cyclodextrin complex.
    Biochimica Biophysica Acta - Biomembranes (2017) 1859:1930-1940

Sandrine Verstraeten (2019)

Contribution of membrane lipids to the activity of the saponin ginsenoside Rh2

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides) (In French)

   

• Publications
  • The activity of the saponin ginsenoside Rh2 is enhanced by the interaction with membrane sphingomyelin but depressed by cholesterol
    Scientific Reports (2019) 9:7285 (14 printed pages)
  • Membrane cholesterol delays cellular apoptosis induced by ginsenoside Rh2, a steroid saponin
    Toxicology and Applied Pharmacology (2018) 18;352:59-67

Tamara Milosevic (2019)

Mitochondrial toxicity induced by oxazolidinone antibiotics: insights from studies in human cell lines and primary cells

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)

   

• Publications
  • Prolonged inhibition and incomplete recovery of mitochondrial function in oxazolidinone-treated megakaryoblastic cell lines
    International Journal of Antimicrobial Agents (2019) 54:661-667
    
  • Mitochondrial alterations (inhibition of mitochondrial protein expression, oxidative metabolism and ultrastructure) induced by linezolid and tedizolid at clinically-relevant concentrations in cultured human HL-60 promyelocytes and THP-1 monocytes
    Antimicrobial Agents and Chemotherapy (2018) 23;62:e01599-17 (17 printed pages)

Ana Miranda Bastos (2019)

Towards optimization of temocillin exposure in haemodialysis patients: a translational approach from bedside-to-bench-to-bedside

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)

   

• Publications
  • Temocillin dosing in haemodialysis patients based on population pharmacokinetics of total and unbound concentrations and Monte Carlo simulations
    Journal of Antimicrobial Chemotherapy (2018) 73:1630-1638
  • Thrice weekly temocillin administered after each dialysis session is appropriate for the treatment of serious Gram-negative infections in haemodialysis patients.
    International Journal of Antimicrobial Agents (2015) 46:660-665
  • Development and validation of a High Performance Liquid Chromatography Assay for the Determination of Temocillin in Serum of Haemodialysis Patients
    Journal of Pharmaceutical and Biomedical Analysis (2014) 90:192–197

Catherine Leonard (2017)

Contribution of plasma membrane lipid domains to red blood cell (re)shaping

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)

   

• Publications
  • Contribution of plasma membrane lipid domains to red blood cell (re)shaping
    Scientific Reports (2017) 7:4264 (16 printed pages)
  • Lipid domains and membrane (re)shaping: from biophysics to biology.
    In: Epand R., Ruysschaert JM. (eds) The Biophysics of Cell Membranes. Springer Series in Biophysics, vol 19. Springer, Singapore
    ISBN 978-981-10-6243-8 - ISBN 978-981-10-6244-5 [eBook]

Ahalieyah Anantharajah (2017)

Contribution to the understanding of Pseudomonas aeruginosa virulence in search of innovative therapeutic approaches: focus on type III secretion system

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)
    
    
• Publications
  • Correlation between cytotoxicity induced by Pseudomonas aeruginosa clinical isolates from acute infections and IL-1-beta secretion in a model of human THP-1 monocytes
    FEMS Pathogens and Disease (2015) 73:ftv049
  • Inhibition of the injectisome and flagellar type III secretion systems by INP1855 impairs Pseudomonas aeruginosa pathogenicity and inflammasome activation
    Journal of Infectious Diseases (2016) 214:1105-1116
  • Targeting Type Three Secretion System in Pseudomonas aeruginosa
    Trends in Pharmacological Sciences (2016) 37:734-749
  • Salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in Pseudomonas aeruginosa by distinct mechanisms
    Antimicrobial Agents and Chemotherapy (2017) 61:e02566-16

Muhammad Hariri Mustafa (2017)

Development of new antibiotic combinations to be administered by dry powder for inhalation to patients with Cystic Fibrosis: microbiological, pharmacological and pharmaceutical studies

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)
    
    
• Publications
  • Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients through Northern Europe
    Antimicrobial Agents and Chemotherapy (2016) 60:6735-6741
  • Acquired resistance to macrolides in Pseudomonas aeruginosa from cystic fibrosis patients
    European Respiratory Journal (2017) 49:1601847 (13 printed pages)

Hussein Chalhoub (2016)

Impact of efflux mechanisms on susceptibility and resistance to beta-lactam antibiotics in Pseudomonas aeruginosa: beyond the usual concepts

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)
    
    
• Publications
  • Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients through Northern Europe
    Antimicrobial Agents and Chemotherapy (2016) 60:6735-6741
  • Avibactam confers susceptibility to a large proportion of ceftazidime-resistant Pseudomonas aeruginosa isolates recovered from cystic fibrosis patients
    Journal of Antimicrobial Chemotherapy (2015) 70:1596-8
  • High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations
    International Journal of Antimicrobial Agents (2016) 48:740-743.
  • Mechanisms of intrinsic resistance and acquired susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients to temocillin, a revived antibiotic
    Scientific Reports (2017) 7:40208 (14 pages)

Nathalie Vandevelde (2014)

Streptococcus pneumoniae infections in a context of Chronic Obstructive Pulmonary Disease (COPD). Study of the factors contributing to the recurrence of the disease.

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defense (slides)
    
    
• Publications
  • Characterization of a collection of Streptococcus pneumoniae isolates from patients suffering from acute exacerbations of chronic bronchitis: in vitro susceptibility to antibiotics and biofilm formation in relation with antibiotic efflux and serotypes/serogroups.
    International Journal of Antimicrobial Agents (2014) 44:209–217
  • Antibiotic activity against naïve and induced Streptococcus pneumoniae biofilms in an in vitro pharmacodynamic model.
    Antimicrobial Agents and Chemotherapy (2014) 58:1348-135
  • Modulation of the activity of moxifloxacin and solithromycin in an in vitro pharmacodynamic model of S. pneumoniae naive and induced biofilms
    Journal of Antimicrobial Chemotherapy (2015) 70:1713-1726

Ann Lismond (2013)

Antimicrobial resistance in Streptococcus pneumoniae isolates from community acquired pneumonia in Belgium, with special reference to efflux mechanism

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defence (slides)
    
    
• Publications
• Antimicrobial susceptibility of Streptococcus pneumoniae isolates from vaccinated and non-vaccinated patients with a clinically-confirmed diagnosis of community-acquired pneumonia (CAP) in Belgium
  International Journal of Antimicrobial Agents (2012) 39:208– 216
• Efflux of novel quinolones in contemporary Streptococcus pneumoniae isolates from community-acquired pneumonia
  Journal of Antimicrobial Chemotherapy (2011) 66:948-951
  

Sophie Denamur (2013)

Intracellular mechanisms of apoptosis induced by aminoglycoside antibiotics

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defence (slides)
    
    
• Publications
• Apoptosis induced by aminoglycosides in LLC-PK1 Cells: comparative study of neomycin, gentamicin, amikacin, and isepamicin using electroporation.
  Antimicrobial Agents and Chemotherapy (2008) 52:2236–2238
• Role of oxidative stress in lysosomal membrane permeabilization and apoptosis induced by gentamicin, an aminoglycoside antibiotic.
  Free Radical Biology & Medicine (2011) 51:1656-1665.

Els Ampe (2013)

Continuous Infusion of Vancomycin in non-ICU Patients: Why, How and What's the Benefit ?

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defence (slides)
    
    
• Publications
• Implementation of a protocol for administration of vancomycin by continuous infusion: pharmacokinetic, pharmacodynamic, and toxicological aspects
  International Journal of Antimicrobial Agents (2013) 41:439-446
• Stability and compatibility of vancomycin for administration by continuous infusion
  Journal of Antimicrobial Chemotherapy (2013) 68:1179-1182

Joseph Lorent (2013)

Membrane interactions of lipopeptides and saponins, two groups of natural amphiphiles

PhD in Biomedical and Pharmaceutical Sciences

Thesis

• Full text
• Public Defence (slides)
  

• Publications
  
  • Induction of Highly Curved Structures in Relation to Membrane Permeabilization and Budding by the Triterpenoid Saponins, alpha- and delta-Hederin.
    Journal of Biological Chemistry (2013) 288:14000-17
  • Effects of surfactin on membrane models displaying lipid phase separation.
    Biochimical Biophysica Acta (2013) 1828:801-15

Laetitia Garcia (2012)

Intracellular pharmacodynamics of antibiotics Against small-colony variants of Staphylococcus aureus In in vitro models of professional phagocytic cells

PhD in Biomedical and Pharmaceutical Sciences

Publications

• Antibiotic activity against Small Colony Variants of Staphylococcus aureus: Review of in vitro, animal, and clinical data
  Journal of Antimicrobial Chemotherapy (2013) 68:1455-1464 (review paper)
• Pharmacodynamic evaluation of the activity of antibiotics against hemin- and menadione-dependent small-colony variants of Staphylococcus aureus in models of extracellular (broth) and intracellular (THP-1 monocytes) infections
  Antimicrobial Agents and Chemotherapy (2012) 56:3700-3711 (Original paper)
• Influence of the protein kinase C activator, phorbol myristate acetate (PMA), on the intracellular activity of antibiotics against hemin and menadione auxotrophic Small-Colony Variant (SCV) mutants of Staphylococcus aureus and their wild-type parental strain in human THP-1 cells
  Antimicrobial Agents and Chemotherapy (2012) 56:6166–6174 (Original paper)
  
• Intracellular forms of menadione-dependent Small-Colony Variants of methicillin-resistant Staphylococcus aureus are hypersusceptible to beta-lactams in a model of THP-1 cells due to cooperation between vacuolar acidic pH and oxidant species
  Journal of Antimicrobial Chemotherapy (2012) 67: 2873–2881 (Original paper)

Coralie Vallet (2012)

Characterization of the active efflux of fluoroquinolones in eukaryotic cells

PhD in Biomedical and Pharmaceutical Sciences

• Thesis
  • Full text
  • Public Defence (slides)
    
    
• Publications
  
  • Characterization of Abcc4 gene amplification in stepwise-selected mouse J774 macrophages resistant to the topoisomerase II inhibitor ciprofloxacin.
    PLoS ONE (2011) 6:e28368 (Original paper)
  • Modulation of the expression of ABC transporters in murine (J774) macrophages exposed to large concentrations of the fluoroquinolone antibiotic moxifloxacin.
    Toxicology (2011) 290:179-187 (Original paper)
  • Cellular accumulation of fluoroquinolones is not predictive of their intracellular activity: studies with gemifloxacin, moxifloxacin, and ciprofloxacin in a pharmacokinetic/pharmacodynamic model of uninfected and infected macrophages.
    International journal of Antimicrobial Agents (2011) 38:249-256. (Original paper)

Hayet Bensikaddour (2011)

Interactions between fluroquinolones and lipids: biophysical studies

PhD in Biomedical and Pharmaceutical Sciences (Orientation: Pharmaceutical Sciences)

• Thesis
  • Full text
  • Public Defence (slides)
    
    
• Publications
  • Interactions of ciprofloxacin with DPPC and DPPG: Fluorescence anisotropy, ATR-FTIR and 31P NMR spectroscopies and conformational analysis
    Biochimica Biophysica Acta (Biomembranes) (2008) 1778:2535-2543 (Original paper)
    
  • Characterization of the interactions between fluoroquinolone antibiotics and lipids: a multitechnique approach
    Biophysical Journal (2008) 94:3035-3046 (Original paper)

Pierre Baudoux (2010)

Antibiotic activity against intracellular Staphylococcus aureus

PhD in Sciences (Doctorat en Sciences)

• Publications
  • Activity of quinupristin-dalfopristin against extracellular and intracellular Staphylococcus aureus with various resistance phenotypes
    Journal of Antimicrobial Chemotherapy (2010) 65:1228-1236 (Original paper)
  • Combined effect of pH and concentration on the activities of gentamicin and oxacillin against Staphylococcus aureus in pharmacodynamic models of extracellular and intracellular infections.
    Journal of Antimicrobial Chemotherapy (2007) 59:246-53 (Original paper)

Sébastien Van de Velde (2010)

Intracellular localization of Listeria monocytogenes: impact on its proteome and antibiotic treatments

PhD in Biomedical and Pharmaceutical Sciences (Doctorat en Sciences Biomédicales et Pharmaceutiques)

• Publications
  • Isolation and 2D-DIGE proteomic analysis of intracellular and extracellular forms of Listeria monocytogenes.
    Proteomics (2009) 9:5484-5496 (Original paper)
  • Penicillin-binding Proteins (PBP) and Lmo0441 (a PBP-like protein) play a role in beta-lactam sensitivity of Listeria monocytogenes.
    Gut Pathogens (2009) 1:23 (Original paper)
  • Contrasting effects of human THP-1 cells differentiation on levofloxacin and moxifloxacin intracellular accumulation and activity against Staphylococcus aureus and Listeria monocytogenes.
    Journal of Antimicrobial Chemotherapy (2008) 62:518-52 (Original paper)

Isabelle Tytgat (2008)

Les DD-ligases comme cibles de nouveaux antibiotiques : Caractérisation de l'activité enzymatique et étude d'inhibiteurs originaux

PhD in Sciences (Doctorat en Sciences)

• Publications
  • DD-Ligases as a Potential Target for Antibiotics: Past, Present and Future.
    Current Medicinal Chemistry (2009) 16: 2566-2580 (Review)
  • Structure-based design of benzoxazoles as new inhibitors for D-alanyl - D-alanine ligase
    QSAR and Combinatorial Sciences (2009) 28: 1394-1404 (Original paper)

Aurélie Olivier (2008)

Intracellular fate of Staphylococcus aureus in human phagocytic and non phagocytic cells and role of virulence factors

PhD in Sciences (Doctorat en Sciences)

• Thesis
  • Full text
    
    
• Publications
  • Role of rsbU and staphyloxanthin in phagocytosis and intracellular growth of Staphylococcus aureus in human macrophages and endothelial cells.
    Journal of Infectious Diseases (2009) 200:1367-1370 (Original paper)
  • Restoration of susceptibility of intracellular Methicillin Resistant Staphylococcus aureus (MRSA) to beta-lactams: comparison between strains, cells, and antibiotics
    Antimicrobial Agents and Chemotherapy (2008) 52:2997-2805 (Original paper)

Sandrine Lemaire (2008)

Intracellular Staphylococcus aureus, an emerging link to persistent and relapsing infections: factors influencing the activity of antimicrobials against intracellular S. aureus

PhD in Biomedical and Pharmaceutical Sciences (Doctorat en Sciences Biomédicales et Pharmaceutiques)

• Thesis
  • Full text
  • Public defence: slides
    
    
• Publications
  • Activities of antistaphylococcal antibiotics towards the extracellular and intraphagocytic forms of S. aureus isolates from a patient with persistent bacteraemia and endocarditis
    Clinical Microbioloy and Infection (2008) 14:766-777 (Original paper)
    
  • Restoration of susceptibility of intracellular Methicillin Resistant Staphylococcus aureus (MRSA) to beta-lactams: comparison between strains, cells, and antibiotics
    Antimicrobial Agents and Chemotherapy (2008) 52:2997-2805 (Original paper)
  • Restoration of susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) to beta-lactam antibiotics by acidic pH: role of penicillin-binding-protein 2A (PBP 2A)
    Journal of Biological Chemistry (2008) 283:12769-12776 (Original paper)
  • Role of Acidic pH in the Susceptibility of Intraphagocytic Methicillin-Resistant Staphylococcus aureus Strains to Meropenem and Cloxacillin
    Antimicrobial Agents and Chemotherapy (2007) 51:1627-1632. (Original paper)
  • Modulation of the Cellular Accumulation and Intracellular Activity of Daptomycin towards phagocytized Staphylococcus aureus by the P-glycoprotein (MDR1) Efflux Transporter in human THP-1 macrophages and Madin-Darby canine kidney cells.
    Antimicrobial Agents and Chemotherapy (2007) 51:2748-275 (Original paper)

Jean-Michel Michot (2007)

Double-edged effect of a MRP- like transporter on pharmacokinetics, pharmacodynamics and toxicodynamics of quinolones antibiotics in macrophages:
involvement of a common efflux pathway

PhD in Biomedical and Pharmaceutical Sciences (Doctorat en Sciences Biomédicales et Pharmaceutiques)

• Publications
  • Cellular accumulation and activity of quinolones in ciprofloxacin-resistant J774 macrophages.
    Antimicrobial Agents and Chemotherapy (2006) 50:1689-1695 (Original paper)
  • Quinolones in 2005: an update
    Clinical Microbiology and Infection (2005) 11:256-280 (Review)
  • Influence of efflux transporters on the accumulation and efflux of four quinolones (ciprofloxacin, levofloxacin, garenoxacin, and moxifloxacin) in J774 macrophages.
    Antimicrobial Agents and Chemotherapy (2005) 49:2429-2437 (Original paper)
  • Active efflux of ciprofloxacin from J774 macrophages through an MRP-like transporter.
    Antimicrobial Agents and Chemotherapy (2004) 48:2673-2682 (Original paper)
  • Antibiotic efflux pumps in eukaryotic cells: occurence and impact on antibiotic cellular pharmacokinetics, pharmacodynamics and toxicodynamics.
    Journal of Antimicrobial Chemotherapy (2003) 51:1067-1077 (Review)

Maritza Barcia-Macay (2007)

Activity of antibiotics against extracellular and intracellular forms of Staphylococcus aureus : Pharmacodynamic studies in vitro using a model of human THP-1 macrophages

PhD in Biomedical and Pharmaceutical Sciences (Doctorat en Sciences Biomédicales et Pharmaceutiques)

• Thesis
  • Full text
  • Public defence: slides
    
    
• Publications
  • Cellular pharmacokinetics of telavancin, a novel lipoglycopeptide antibiotic, and analysis of lysosomal changes in cultured eukaryotic cells (J774 mouse macrophages; rat embryonic fibroblasts).
    Journal of Antimicrobial Chemotherapy (2008) 61:1288-1294 (Original paper)
  • Evaluation of the Extracellular and Intracellular Activities (human THP-1 macrophages) of Telavancin vs. Vancomycin against Methicillin-susceptible, Methicillin-resistant, Vancomycin-intermediate and Vancomycin-resistant Staphylococcus aureus
    Journal of Antimicrobial Chemotherapy (2006) 58:1177–1184 (Original paper)
  • Pharmacodynamic evaluation of the intracellular activity of antibiotics against Staphylococcus aureus in a model of THP-1 macrophages.
    Antimicrobial Agents and Chemotherapy (2006) 50:841-8 (Original paper)

Anne Spinewine (2006)

Appropriate use of medicines in care of the elderly: factors uderlying inapproriateness, and impact of the clinical pharmacist

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Thesis
  • Full text (2.6 MB)
  • Public defence: slides (1.5 MB)
    
    
• Publications
  • Implementation of ward-based clinical pharmacy services in Belgium - Description of the impact on a geriatric unit.
    Annals of Pharmacotherapy (2006) 40:720-728
  • Appropriateness of use of medicines in elderly inpatients: qualitative study
    British Medical Journal (2005) 331:935-940
  • La pharmacie clinique, une nouvelle orientation pharmaceutique au service des patients: réalisations à l'étranger et possibilités en Belgique.
    Louvain Médical (2003) 122:127-139

Helène Servais (2006)

Lysosomes, weapons or shield in gentamicin-induced apoptosis?

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Thesis
  • Full text: high resolution (16 MB) - low resolution (9MB)
  • Public defecse: slides (7 MB)
    
    
• Publications:
  • Antibiotic-induced nephrotoxicity
    In: Toxicology of the Kidney (Target Organ Toxicology Series), 2004, chap. 16, pp 635-685, J.B. Tarloff & L.H. Lash, eds., CRC Press, Boca Raton, Fla. (Review)
  • Gentamicin-induced apoptosis in LLC-PK1 cells: involvement of lysosomes and mitochondria
    Toxicology and Applied Pharmacolology (2005) 15:321-333 (Original paper)
  • Gentamicin causes apoptosis at low concentrations in renal LLC-PK1 cells subjected to electroporation
    Antimicrobial Agents and Chemotherapy (2006) 50:1213-122 (Original paper)

Stéphane Carryn (2003)

Control of intracellular Listeria monocytogenes growth by antibiotics and granulocyte-macrophage colony stimulating factor

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Publications
  • Comparative intracellular (THP-1 Macrophage) and extracellular activities of beta-lactams, azithromycin, gentamicin, and fluoroquinolones against Listeria monocytogenes at clinically relevant concentrations
    Antimicrobial Agents and Chemotherapy (2002) 46:2095-2103 Original paper
  • Intracellular pharmacodynamics of antibiotics
    Infectious Disease Clinics of North America (2003) 17:615-634 (Review)
  • Activity of beta-lactams (ampicillin, meropenem), gentamicin, azithromycin and moxifloxacin against intracellular Listeria monocytogenes in a 24h THP-1 human macrophage model
    Journal of Antimicrobial Chemotherapy (2003) 51:1051-1052 (Original paper)
  • Impairment of growth of Listeria monocytogenes in THP-1 macrophages by granulocyte macrophage colony-stimulating factor : release of tumor necrosis factor-a and nitric oxide
    The Journal of Infectious Diseases (2004) 189:2101-2109 (Original paper)

Donatienne Tyteca (2002)

Azithromycin, a pharmacological agent which selectively inhibits some pathways of endocytosis: characterization, interests and mechanism of action

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Thesis
  • Full text: high resolution (18 Mb) | low resolution (3.4 Mb)
  • Public defence: Slides (HTML and PDF)
    
    
• Publications:
  • Azithromycin, a lysosomotropic antibiotic, impairs fluid-phase pinocytosis in cultured fibroblasts
    European Journal of Cell Biology (2001) 80:466-478 (Original paper)
  • Azithromycin, a lysosomotropic antibiotic, has distinct effects on fluid-phase and receptor-mediated endocytosis, but does not impair phagocytosis in J774 macrophages
    Experimental Cell Research (2002) 281:86-100 (Original paper)
  • The macrolide antibiotic azithromycin ineracts with lipids and affects membrane organization and fluidity: studies on Langmuir-Blodgett monolayers, liposomes and J774 macrophages
    Journal of Membrane Biology (2003) 192:203-215 (Original paper)

Mohammed El Mouedden (2000)

Mise en évidence et étude de l'apoptose induite par les antibiotiques du groupe des aminoglycosides

PhD in Biomedical Sciences (Doctorat en Sciences Biomédicales - orientation: biologie et pharmacologie cellulaires)

• Thesis
  • slides
    
    
• Publications
  • Apoptosis in renal proximal tubules of rats treated with low doses of aminoglycosides.
    Antimicrobial Agents and Chemotherapy (2000) 44:665-675 (Original paper)
  • Gentamicin-induced apoptosis in renal cell lines and embryonic rat fibroblasts.
    Toxicological Sciences (2000) 56:229-239 (Original paper)

Isabelle Paternotte-Henry (2000)

Conception, synthèse et évaluation de prodrogues esters basiques d'ampicilline en vue d'une action microbicide intracellulaire

PhD in Sciences (Doctorat en Sciences - orientation: chimie)

• Publication
  • Ester prodrugs of ampicillin tailored for intracellular accumulation.
    Bioorg Med Chem Lett (1997) 7:3107-311 (Original paper)

PhD in Sciences (Doctorat en Sciences - orientation: biologie)

• Publications
  • Lysosomal alterations induced in cultured rat fibroblasts by long-term exposure to low concentrations of azithromycin.
    J Antimicrob Chemother (1998) 42:761-767 (Original paper)
  • Hyperactivity of cathepsin B and other lysosomal enzymes in fibroblasts exposed to azithromycin, a dicationic macrolide antibiotic with exceptional tissue accumulation.
    FEBS Lett (1996) 394:307-310 (Original paper)

Modulation de l'infection intracellulaire à Listeria monocytogenes et de l'activité intrinsèque des antibiotiques par l'interféron gamma et l'interleukine 6

PhD in Biomedical Sciences (Docteur en Sciences Biomédicales - orientation: Biologie et pharmacologie cellulaires)

• Publications
  • Modulation of intracellular growth of Listeria monocytogenes in human enterocyte-like Caco-2 cells by interferon-gamma and interleukin-6: role of nitric oxide and cooperation with antibiotics.
    J Infect Dis (1999) 180:1195-1204 (Original paper)
  • Mechanism of the intracellular killing and modulation of antibiotic susceptibility of Listeria monocytogenes in THP-1 macrophages activated by gamma-interferon.
    Antimicrob Agents Chemother (1999) 43:1242-1251(Original paper)

Etudes des surcharges lysosomiales induites par l'azithromycine dans des cellules en culture: effets sur les phospholipides et le cholestérol cellulaires

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Publications
  • Interactions of macrolide antibiotics (erythromycin A, roxithromycin, erythromycylamine and azithromycin) with phospholipids: computer-aided conformational analysis and studies on acellular and cell culture models.
    Toxicol Applied Pharmacol (1999) 156:129-140 (Original paper)
  • Interaction of the macrolide azithromycin with phospholipids. II. Biophysical and computer-aided conformational studies.
    Eur J Pharmacol (1996) 314:215-22 (Original paper)
  • Interaction of the macrolide azithromycin with phospholipids. I. Inhibition of lysosomal phospholipase A1 activity.
    Eur J Pharmacol (1996) 314:203-214. (Original paper)

Influence des antibiotiques du groupe des aminoglycosides sur les propriétés membranaires: études sur liposomes

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Publications
  • Aminoglycoside antibiotics prevent the formation of non bilayer structures in negatively-charged membranes. Comparative studies using fusogenic [bis-(beta-diethylaminoethylether)hexestrol] and aggregating (spermine) agents.
    Chem Phys Lipids (1996) 79:123-135. (Original paper)
  • Aminoglycoside antibiotics induce aggregation but not fusion of negatively-charged liposomes.
    Eur J Pharmacol (1995) 289:321-333. (Original paper)
  • Alterations in membrane permeability induced by aminoglycoside antibiotics: studies on liposomes and cultured cells.
    Eur J Pharmacol (1993) 247:155-168. (Original paper)

Conception de modèles quantitatifs d'infection intracellulaire pour l'étude de l'action des antibiotiques

PhD in Biomedical Sciences (Doctorat en Sciences Biomédicales - orientation: biologie et pharmacologie cellulaires)

• Publication
  • Effect of recombinant human gamma interferon on intracellular activities of antibiotics against Listeria monocytogenes in the human macrophage cell line THP-1.
    Antimicrob Agents Chemother (1996) 40:1225-1230. (Original paper)

In vitro models for the study of the regulation of the activity of lysosomal phospholipase A1 by membrane lipids and of its inhibition by aminoglycoside antibiotics

PhD in Sciences (Doctorat en Sciences - orientation: chimie)

• Publication
  • Effect of substrate organization on the activity and on the mechanism of gentamicin-induced inhibition of rat liver lysosomal phospholipase A1.
    Biochem Pharmacol (1992) 43:895-898. (Original paper)

Evaluation systématique et prospective du potentiel toxique de nouveaux aminoglycosides

PhD in Biomedical Sciences (Sciences Biomédicales)

The mechanism of aminoglycoside binding to isolated brush border membrane of proximal tubular cells

PhD in Biomedical Sciences (Doctorat en Sciences Biomédicales - orientation: biologie et pharmacologie cellulaires)

Renal phospholipidosis and phospholipiduria induced by aminoglycoside antibiotics: animal and human studies

PhD in Biomedical Sciences (Doctorat en Sciences Biomédicales: orientation: biologie et pharmacologie cellulaires)

• Publications
  • Urinary phospholipids excretion in neonates treated with amikacin.
    Int J Clin Pharm Res (1994) 14:149-156. (Original paper)
  • Once-a-day administration of amikacin in neonates: assessment of nephrotoxicity and ototoxicity.
    Dev Pharmacol Ther (1993) 20:220-230. (Original paper)

Polyanionic compounds as protectants against aminoglycoside-induced nephrotoxicity

PhD in Biomedical Sciences (Doctorat en Sciences Biomédicales: orientation: biologie et pharmacologie cellulaires)

• Publications
  • Comparative assessment of poly-L-aspartic and poly-L-glutamic acids against gentamicin-induced renal lysosomal phospholipidosis, phospholipiduria and cell proliferation in rats.
    J Pharmacol Exp Ther (1992) 262:424-432 (Original paper)
  • Mechanism of protection afforded by polyaspartic acid against gentamicin-induced phospholipidosis. I. Polyaspartic acid binds gentamicin and displaces it from negatively charged phospholipid layers in vitro.
    J Pharmacol Exp Ther (1990) 255:867-874.(Original paper)
  • Mechanism of protection afforded by polyaspartic acid against gentamicin-induced phospholipidosis. II. Comparative in vitro and in vivo studies with poly-L-aspartic, poly-L-glutamic and poly-D-glutamic acids.
    J Pharmacol Exp Ther (1990) 255:875-885 (Original paper)

Construction, characterization and evaluation of the cellular delivery of diptheria toxin fragment B - associated liposomes containing ribavirin

PhD in Sciences (Doctorat en Sciences - orientation: biologie)

Marie-Paule Mingeot-Leclercq (1988)

Mécanisme moléculaire de la néphrotoxicité induite par les aminoglycosides : études biochimiques et biophysiques

PhD in Pharmaceutical Sciences (Doctorat en Sciences Pharmaceutiques)

• Publications
  • Effect of acidic phospholipids on the activity of lysosomal phospholipases and on their inhibition by aminoglycoside antibiotics. I. Biochemical analysis.
    Biochem Pharmacol (1990) 40:489-497. (Original paper)
  • Effect of acidic phospholipids on the activity of lysosomal phospholipases and on their inhibition by aminoglycoside antibiotics. II. Conformational analysis.
    Biochem Pharmacol (1990) 40:499-506. (Original paper)
  • Ultrastructural, physico-chemical and conformational study of the interactions of gentamicin and bis(beta-diethylaminoethylether)hexestrol with negatively charged phospholipid bilayers.
    Biochem Pharmacol (1989) 38:729-741. (Original paper)
  • Biochemical mechanism of aminoglycoside-induced inhibition of phosphatidylcholine hydrolysis by lysosomal phospholipases.
    Biochem Pharmacol (1988) 37:591-599. (Original paper)

Mise au point de modèles pour l'étude de dérivés lysosomotrophes de beta-lactames

PhD in Sciences (Doctorat en Sciences - orientation: biologie)

• Publications
  • Coupling products of amino acids to penicillin V and cephalothin: synthesis, and susceptibility to carboxypeptidases and lysosomal enzymes.
    J Med Chem (1988) 31:976-983. (Original paper)
  • Influence of conversion of penicillin G into a basic derivative on its accumulation and subcellular localization in cultured macrophages.
    Antimicrob Agents Chemother (1987) 31:410-416. (Original paper)

Interactions entre les aminoglycosides et les structures subcellulaires du cortex renal chez le rat: études biochimiques et morphologiques à doses faibles

PhD in Applied Biomedical Sciences (Doctorat en Sciences Biomédicales Appliquées)

• Publication
  • Impairment of lysosome-pinocytic vesicle fusion in rat kidney proximal tubules after treatment with gentamicin at low doses.
    Toxicol Appl Pharmacol (1986) 86:271-285. (Original paper)

Marie-Béatrice Carlier (1984)

La phospholipidose rénale induite par les aminoglycosides: étude biochimique et morphologique

PhD in Applied Pharmaceutical Sciences  (Doctorat en Sciences Pharmaceutiques Appliquées)

• Publication
  • Inhibition of lysosomal phospholipases by aminoglycoside antibiotics: in vitro comparative studies.
    Antimicrob Agents Chemother (1983) 23:440-449. (Original paper)
  • Mechanism of aminoglycoside-induced lysosomal phospholipidosis: in vitro and in vivo studies with gentamicin and amikacin.
    Biochem Pharmacol (1982) 31:3861-3870 (Original paper)

Implication des hémolysines de S. aureus dans le développement de l'infection intracellulaire dans une lignée de cellules phagocytaires

• Slides

Activité des quinolones vis-à-vis des formes extracellulaires et intracellulaires de Listeria monocytogenes : modulation par les pompes à efflux bactériennes et eucaryotes

• Slides
• Publication (Antimicrob. Agents Chemother.)

Perfusion continue des beta-lactames: 1. Etude de la compatibilité du céfépime en vue de son administration par perfusion continue; 2. Etude clinique comparative de la perfusion continue et de l'administration conventionnelle chez les patients atteints de pneumonie nosocomiale (étude pharmacocinétique, pharmacodynamique et pharmacoéconomique).

• Publication (J. Antimicrob. Chemother.)

Etude de la stabilité et de la compatibilité de la ceftazidime en vue de son administration par perfusion continue chez des patients atteints de pneumopathies nosocomiales

• Publication (Antimicrob. Agents Chemother.)
  

Pharmacocinétique des antibiotiques dans des populations particulières: validation de méthodes analytiques et études pharmacocinétiques

• Poster
• Publication (Clinical Biochemistry)

Etude de la pharmacodynamie et pharmacocinétique cellulaire d’un nouvel antibiotique : le GSK1322322

• Poster

Influence de la nicotine sur la formation de biofilm par S. pneumoniae et l'activité des antibiotiques

• Poster

Etudes pharmacologiques de l'avibactam, un nouvel inhibiteur de beta-lactamase à large spectre

• Poster
• Publication (J. Antimicrob. Chemother).

Pharmacocinétique et toxicité cellulaire des oxazolidinones, une nouvelle classe d'antibiotiques

• Poster

Infections à Streptococcus pneumoniae dans un contexte de broncho-pneumopathie chronique obstructive. Etude de l'effet du diazépam et de la lévomépromazine sur la susceptibilité aux fluoroquinolones

• Poster
  

Influence du Panobacumab, un anticorps IgM monoclonal, sur la phagocytose de Pseudomonas aeruginosa par les cellules THP-1

• Poster

Etude de l'activité de la ceftaroline, une nouvelle céphalosporine anti-MRSA, vis-à-vis de formes extracellulaires et intracellulaires de Staphylococcus aureus.

• Poster
• Publication (J. Antimicrob. Chemother.)

• Poster

Toxicité des fluoroquinolones et des inhibiteurs de topoisomérases I et II dans des cellules exposées de façon chronique aux fluoroquinolones

• Publication (Toxicology)

Rôle des transporteurs multidrogues (MDR) dans l'accumulation cellulaire d'antibiotiques dans les macrophages J774

• Poster
• Publication (Int J. Antimicrob. Ag.)

L’efflux actif comme mécanisme d’interactions médicamenteuses : Compétition entre Fluoroquinolones et Antiviraux dans un modèle de macrophages J774

• Full text
• Slides
• Oral presentation

Implication des transporteurs ABC dans l’efflux de médicaments au niveau des cellules eucaryotes.

• Poster

• Slides

Apoptose : un marqueur de toxicité des aminoglycosides? Étude comparative sur cellules en culture incubées avec de la gentamicine et de l’amikacine.

• Slides
• Publication

L’efflux actif comme mécanisme d’interactions médicamenteuses : compétition entre fluoroquinolones et AINS pour un même transporteur dans un modèle de macrophages J774

• Slides
• Poster

• Publication (J. Antimicrob. Chemother.)

• Publication (J. Antimicrob. Chemother.)

Accumulation et efflux des fluoroquinolones dans des macrophages J774 sauvages et résistants à la ciprofloxacine

• Slides
• Publication (Antimicrob.Agents Chemother.)

• Full text (PDF)
  
• Publication (J. Antimicrob. Chemother.)

Influence du glatiramer sur l'infection intracellulaire des THP-1 à Listeria monocytogenes et sur l'activité des antibiotiques

• Publication (J. Antimicrob. Chemother.)