Effet de Bcl-2 sur l’activité des caspases induite par la gentamicine dans les cellules MDCK a

gentamicin-treated cellsb

bcl-2 (+) bcl-2 (-)

controls cells c untransfected cells transfectants d transfectants e

266.9 ? 11.4 444.4 ± 11.6 * 290.3 ± 5.2 410.6 ± 12.9 *

a using DEVD-AMC as a substrate (this substrate is cleaved by caspases 3, 6, 7 and 8)

b cells incubated for 4 days with 2 mM gentamicin

c untransfected cells not exposed to gentamicin

d cells transfected with a bcl-2 gene-containing vector

e cells transfected with a control vector

Statistical analysis:

* significantly larger than control cells (p < 0.001) and than bcl-2(+) transfected cells (p < 0.001)