The AAP (3’)-IIIa enzyme shows commonality with the eucaryotic protein kinase
The crystal structure of the aminoglycoside kinase, APH(3')-IIIa, which catalyzes the phosphorylation of a broad spectrum of aminoglycoside antibiotics, was determined at 2.2 Å. resolution.
The three-dimensional fold of APH(3')-IIIa reveals a striking similarity to
eukaryotic protein kinases despite a virtually complete lack of sequence
These results indicate that APH(3') enzymes and eukaryotic-type protein kinases share a common ancestor.
Cell 1997 Jun 13;89(6):887-95
Structure of an enzyme required for aminoglycoside antibiotic resistance reveals
homology to eukaryotic protein kinases.
Hon WC, McKay GA, Thompson PR, Sweet RM, Yang DS, Wright GD, Berghuis AM
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.