What is the place of pharmacological inhibitors to dissect the endocytic apparatus ?
Conditions, mutations and agents have been extensively used to dissect cellular mechanisms of endocytosis:
? K+ depletion (Cupers et al, 1994)
? incubation in hypertonic medium (Cupers et al, 1994; 1997)
? cytosol acidification (Sandvig et al, 1987)
? clathrin, adaptor proteins and associated proteins
(for a review, see Dautry-Varsat, 2001)