Saccharomyces boulardii upgrades cellular adaptation after proximal enterectomy in rats.
Buts JP, De Keyser N, Marandi S, Hermans D, Sokal EM, Chae YH, Lambotte L, Chanteux H, Tulkens PM
Laboratory of Paediatric Gastroenterology and Nutrition, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
BACKGROUND: Saccharomyces boulardii is a non-pathogenic yeast which
exerts trophic effects on human and rat small intestinal mucosa.
AIMS: To examine the effects of S boulardii on ileal adaptation after
proximal enterectomy in rats. METHODS: Wistar rats, aged eight weeks, underwent
60% proximal resection or transection and received by orogastric intubation
either 1 mg/g body wt per day lyophilised S boulardii or the vehicle for
seven days. The effects on ileal mucosal adaptation were assessed eight
days after surgery.
RESULTS: Compared with transection, resection resulted in mucosal hyperplasia
with significant decreases in the specific and total activities of sucrase,
lactase, and maltase. Treatment of resected animals with S boulardii had
no effect on mucosal hyperplasia but did upgrade disaccharidase activities
to the levels of the transected group. Enzyme stimulation by S boulardii
was associated with significant increases in diamine oxidase activity and
mucosal polyamine concentrations. Likewise, sodium dependent D-glucose
uptake by brush border membrane vesicles, measured as a function of time
and glucose concentration in the incubation medium, was significantly (p<0.05)
increased by 81% and three times respectively in the resected group treated
with S boulardii. In agreement with this, expression of the sodium/glucose
cotransporter-1 in brush border membranes of resected rats treated with
S boulardii was enhanced twofold compared with resected controls.
CONCLUSION: Oral administration of S boulardii soon after proximal
enterectomy improves functional adaptation of the remnant ileum.
PMID: 10369710, UI: 99298222