1. New Microbiol. 2017 Jul;40(3):165-169. Epub 2017 May 17.

Magnetic nanoparticle-enhanced PCR for the detection and identification of
Staphylococcus aureus and Salmonella enteritidis.

Houhoula D(1), Papaparaskevas J(2), Zatsou K(1), Nikolaras N(1), Malkawi HI(3),
Mingenot-Leclercq MP(4), Konteles S(1), Koussisis S(1), Tsakris A(2), Charvalos
E(5).

Author information: 
(1)Department of Food Technology, Technological Educational Institution of
Athens, 12210, Athens, Greece.
(2)Department of Microbiology, Medical School, National and Kapodistrian
University of Athens, 11527, Athens, Greece.
(3)Department of Biological Sciences, Yarmouk University, Irbid-Jordan, Jordan.
(4)Louvain Drug Research Institute, Université catholique de Louvain, B-1200,
Brussels, Belgium.
(5)Central Labs, Iaso Hospital, 12152, Greece.

This paper evaluated magnetic nanoparticle-enhanced PCR for the detection and
identification of Staphylococcus aureus and Salmonella enteritidis. Two different
types of magnetic nanoparticles designated MPIO (iron concentration 2.5 mg/ml,
size 1 µm) and NP (iron concentration 8.7 mg/ml, size 60 nm), both conjugated
with S. aureus or S. enteritidis antibodies were evaluated as an enrichment
procedure for PCR-detection of the pathogens in Trypticase Soy Broth, milk, blood
and meat broth. Bacterial suspensions (1.5x108 cfu/ml) were prepared and serial
diluted 10-1. The MPIO and NP nanoparticles were added, followed by incubation
for 1 hour at room temperature, magnetic separation of the pellet, DNA extraction
and PCR, targeting the femA and invA sequences. The nanoparticle-free and the
NP-supplemented dilutions were positive down to the 1.5x102 cfu/ml concentration 
for both bacteria. The MPIO-supplemented dilutions were positive down to approx. 
2x100 cfu/ml concentration, respectively. Bacteria-free TSB was negative by PCR. 
MPIO nanoparticles (size 1 µm) enhanced the detection of S. aureus and S.
enteritidis by PCR, whilst NP nanoparticles (size 60 nm) did not, thus indicating
that the size of the magnetic nanoparticles play a significant role in the
enrichment procedure.


PMID: 28513808