1. J Control Release. 2018 Dec 28;292:248-255. doi: 10.1016/j.jconrel.2018.11.005.
Epub 2018 Nov 5.

The origin of neural stem cells impacts their interactions with targeted-lipid
nanocapsules: Potential role of plasma membrane lipid composition and fluidity.

Carradori D(1), Dos Santos AG(2), Masquelier J(3), Paquot A(3), Saulnier P(4),
Eyer J(4), Préat V(5), Muccioli GG(3), Mingeot-Leclercq MP(2), des Rieux A(6).

Author information: 
(1)Université catholique de Louvain, UCLouvain, Louvain Drug Research Institute, 
Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels,
Belgium; Université d'Angers, Unité de Micro et Nanomédecines Translationelles
(UMR INSERM 1066/CNRS 6021), Institut de Biologie en Santé PBH-IRIS, CHU Angers, 
49033 Angers, France.
(2)Université catholique de Louvain, UCLouvain, Louvain Drug Research Institute, 
Cellular and Molecular Pharmacology, Avenue E. Mounier 73, 1200 Brussels,
Belgium.
(3)Université catholique de Louvain, UCLouvain, Louvain Drug Research Institute, 
Bioanalysis and Pharmacology of Bioactive Lipids, Avenue E. Mounier 72, 1200
Brussels, Belgium.
(4)Université d'Angers, Unité de Micro et Nanomédecines Translationelles (UMR
INSERM 1066/CNRS 6021), Institut de Biologie en Santé PBH-IRIS, CHU Angers, 49033
Angers, France.
(5)Université catholique de Louvain, UCLouvain, Louvain Drug Research Institute, 
Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels,
Belgium.
(6)Université catholique de Louvain, UCLouvain, Louvain Drug Research Institute, 
Advanced Drug Delivery and Biomaterials, Avenue E. Mounier 73, 1200 Brussels,
Belgium. Electronic address: anne.desrieux@uclouvain.be.

The adsorption of a peptide (NFL-TBS.40-63 peptide (NFL)) known to induce neural 
stem cells (NSC) differentiation in vitro, at the surface of lipid nanocapsules
(LNC) provides a targeting drug delivery system (NFL-LNC) that penetrates
subventricular zone-neural stem cells (SVZ-NSC) but not central canal-NSC
(CC-NSC). We hypothesized preferential interactions could explaine, at least
partially, the different properties of SVZ- and CC-NSC plasma membranes. The
objective of this work was to compare SVZ- and CC-NSC plasma membrane lipid
composition, fluidity and permeability. Plasma membranes of SVZ- and CC-NSC were 
isolated and analyzed by LC-MS for their lipid content. Membrane fluidity was
evaluated by measuring the generalized polarization (GP) of Laurdan and membrane 
permeability by fluorescent dextran penetration. Liposomes with different lipid
compositions and steady state fluidities were prepared. ΔGP was measured after
incubation with NFL-LNC. A significantly higher proportion of cholesterol,
ceramides, sphingomyelins, phosphatidylethanolamines and a lower proportion of
phosphatidylcholines and sulfatides were observed in SVZ- compared to CC-NSC.
Fluidity, probably more than lipid composition, drove NFL-LNC and NSC
interactions, and SVZ-NSC were more sensitive to NFL permeabilization than
CC-NSC. We demonstrated that NSC membrane lipid composition and fluidity depended
of NSC origin and that these features could play a role in the specific
interactions with NFL-LNC.

Copyright © 2018 Elsevier B.V. All rights reserved.

DOI: 10.1016/j.jconrel.2018.11.005 
PMID: 30408552