Probing peptidemembrane interactions using AFM
Robert Brasseur, Magali Deleu, Marie-PauleMingeot-Leclercq, Grégory
Francius and Yves F. Dufrêne
Surf. Interface Anal. 2008; 40: 151156
Atomic forcemicroscopy (AFM) has become a powerful addition to the range of
instruments available to probe the organization
of lipid monolayers and bilayers. Currently, AFM is the only tool that can provide
nanoscale topographic images of supported
lipid membranes under physiological conditions, enabling researchers to resolve
their detailed structure and to monitor their
interaction with drugs, peptides and proteins. Here, we survey recent data obtained
by our research groups that demonstrate
the power of the technique for exploring peptidemembrane interactions,
with an emphasis on microbial lipopeptides and on
tilted peptides.
Copyrightc 2008 John Wiley & Sons, Ltd.
Keywords: AFM; membranes; nanoscale resolution; peptides; real-time imaging; supported lipid films